實(shí)驗(yàn)?zāi)康?/strong>
對(duì)擬南芥或水稻穗進(jìn)行研磨破碎,進(jìn)而提取其DNA。
實(shí)驗(yàn)原理
通過研磨珠的撞擊,充分?jǐn)M南芥或水稻穗細(xì)胞,使其內(nèi)容物流出,進(jìn)而提取目標(biāo)成分。
實(shí)驗(yàn)材料和器具
樣品:擬南芥或水稻穗
儀器:全自動(dòng)樣品冷凍研磨儀(上海凈信,Tissuelyser-48)
耗材:離心管,不銹鋼研磨珠(上海凈信)
試劑:TPS溶液(100mM Tris-Cl (pH 8.0),10mM EDTA (pH 8.0),1M KCl)
實(shí)驗(yàn)步驟
需了解具體步驟,請(qǐng)致電廠家
實(shí)驗(yàn)注意事項(xiàng)
1.-20攝氏度下該方法的DNA可以保存4個(gè)月以上,4°大致可以放2-3周。
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