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sticky-Slide Chemotaxis

Model:80328 10812

Brand:ibidi

Specifications :10 (individually packed)

Introduction:A chemotaxis bottomless slide used in 2D and 3D applications with a self-adhesive underside designed to allow self-assemblage of the slide setup Versatile – i.e., permits the mounting of a variety of bottom materials

Applications:

Inserting materials or tissue into chemotaxis chambers

Keeping cell spheroids or tissue in a stable concentration gradient

Chemotaxis of fast or slow migrating cells on either a 2D surface or in 3D gels

Usable with a range of bottom materials, such as plastic sheets, silicon chips, and glass slides

Specifications:

Outer dimensions (w x l)25.5 x 75.5 mm2
Chemotaxis chambers on slide3
Volume per chamber130 μl
Observation area2 x 1 mm2
Height of reservoirs1.05 mm
Total height with plugs12 mm
Volume chemoattractant30 μl
Bottomnone


Technical Features:

Bottomless slide

Self-adhesive underside

Biocompatible adhesive that has been cell culture tested

Adheres to all flat surfaces, even wet surfaces

Suitable coverslips available

Specifications identical to those of the μ-Slide Chemotaxis, except that it has no bottom

Placing Tissue Sample in the sticky-Slide Chemotaxis

Application Example: Directed Invasion of MCF-7 Cancer Cells in an FCS Gradient

This movie shows the invasion of MCF-7 cancer cells into a collagen gel during 48 hours. The center channel of a sticky-Slide Chemotaxis was increased in height by milling in order to position the MCF-7 spheroid. The left side contains 10% fetal calf serum, the right side is serum-free. Phase contrast microscopy, objective lens 4x.

Technical Drawing

Technical drawings and details are available in the Instructions (PDF).

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