樟樹(shù)是一種用途很廣的樹(shù)種,在我國(guó)資源非常豐富,藥用價(jià)值和綠化價(jià)值非常高。主要分布于我國(guó)南方各省,尤以福建臺(tái)灣為最多。具有祛風(fēng),除濕,解毒,殺蟲(chóng)之功效。主治風(fēng)濕痹痛,胃痛,水火燙傷,瘡瘍腫毒,慢性下肢潰瘍,疥癬,皮膚瘙癢等。
近年來(lái),國(guó)內(nèi)外學(xué)者在樟樹(shù)的化學(xué)成分和生物活性方面做了一些研究。研究發(fā)現(xiàn)樟樹(shù)含有化學(xué)成分主要包括揮發(fā)性油、生物堿類、黃酮類、有機(jī)酸等。并且發(fā)現(xiàn)樟樹(shù)的莖、葉、根樟樹(shù)籽等各部分提取物具有一定的生物活性,主要涉及抑菌、驅(qū)蟲(chóng)、抗氧化、消炎等方面。
然而,這些方面的研究還不夠系統(tǒng),有必要對(duì)樟樹(shù)葉的化學(xué)成分和生物活性進(jìn)行更系統(tǒng)全面的研究。
下面內(nèi)容講述廣東省林科院科研團(tuán)隊(duì)研磨樟樹(shù)葉片前處理的方法步驟:
一、實(shí)驗(yàn)儀器多樣品組織研磨儀Tissuelyser-48L(下圖所示)
二、上海凈信®的Tissuelyser®組織研磨儀(專利產(chǎn)品,仿冒必究!ZL2019301641779)是一種特殊的、快速的、高效率的、多試管的一致系統(tǒng)。它能將任何來(lái)源(包括土壤、植物和動(dòng)物的組織/器官、細(xì)菌、酵母、真菌、孢子、古生物標(biāo)本等)的原始DNA、RNA和蛋白質(zhì)進(jìn)行提取和純化。Tissuelyser-48L采用垂直振蕩方式,研磨更充分,1分鐘內(nèi)完成2×24、2×48、4×96個(gè)樣品的研磨;同一組織樣本設(shè)定相同程序,獲得相同的研磨效果;內(nèi)置程序控制器,可對(duì)研磨時(shí)間、轉(zhuǎn)子的振動(dòng)頻率等參數(shù)進(jìn)行設(shè)置;具備安全罩及安全鎖;無(wú)交叉污染且低噪音。
實(shí)驗(yàn)步驟:
將樟樹(shù)葉片剪小段后放入若干ML研磨罐中,加入特定規(guī)格的研磨介質(zhì),然后把研磨罐放入液氮眾泡制幾分鐘,泡制至超低溫后放進(jìn)上海凈信多樣品研磨儀內(nèi),設(shè)置好研磨儀工作時(shí)間和頻率,最后啟動(dòng)設(shè)備。
實(shí)驗(yàn)結(jié)果:
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