實驗?zāi)康?先天殘疾人群人體組織代謝組學技術(shù)應(yīng)用、
實驗地點: 麥特繪譜生物科技(上海)有限公司杭州重點實驗室
實驗材料和器具:
1、樣品:人體軟骨組織,如:耳軟骨、肋軟骨 2、儀器:全自動快速研磨儀(上海凈信)、鋼罐及配套適配器和鋼珠
實驗步驟:
1、研磨罐及研磨珠預(yù)處理(根據(jù)老師需求處理好);
2、將盛裝肋軟骨的凍存管從冰箱取出放入液氮內(nèi)預(yù)冷凍,將處理好的鋼罐及鋼珠放入液氮內(nèi)預(yù)冷凍;
3、預(yù)冷凍過后迅速將凍存管內(nèi)的樣品轉(zhuǎn)移到預(yù)冷凍過的鋼管內(nèi)蓋好再放入液氮內(nèi)冷凍;
4、設(shè)定好運行參數(shù),迅速將液氮冷凍好的樣品罐放如適配器中;
5、啟動儀器至研磨程序結(jié)束,可得到如圖所示樣品;
6、取出樣品罐轉(zhuǎn)移樣品至離心管待分析。
全自動樣品快速研磨儀研磨人體軟骨組織的實驗結(jié)果:
Previous:全自動樣品研磨儀研磨土壤中微生物破壁、提取-上海凈信
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