實(shí)驗(yàn)?zāi)康?/strong>
研磨破碎多酚含量高的植物(木本植物),后續(xù)可提取其核酸或蛋白。
實(shí)驗(yàn)原理
充分磨碎研磨破碎多酚含量高的植物,再用相關(guān)試劑提取其總RNA。
實(shí)驗(yàn)材料和器具
樣品:銀杏葉子
儀器:高通量組織研磨儀(上海凈信,Tissuelyser-24)
耗材:離心管,研磨珠
實(shí)驗(yàn)步驟
需了解具體步驟,請(qǐng)致電廠家
實(shí)驗(yàn)注意事項(xiàng)
如果需要提取的樣品比較多,研磨好的樣品在后續(xù)的提取中,需要轉(zhuǎn)移到大一點(diǎn)的epp管中,研磨時(shí)可以加入較少的抽提液,研磨完畢按照樣品的體積去提取液補(bǔ)充抽提液,進(jìn)行后續(xù)的操作。
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