實驗?zāi)康?/strong>
對大腸桿菌進行破壁,提取其蛋白質(zhì)。
實驗原理
通過研磨珠的撞擊,破碎大腸桿菌的細胞壁,使其內(nèi)容物流出,進而提取其蛋白質(zhì)。
實驗材料和器具
樣品:大腸桿菌
儀器:全自動樣品冷凍研磨儀(上海凈信,JXFSTPRP-96)
耗材:離心管,研磨珠(上海凈信)
實驗步驟
需了解具體步驟,請致電廠家
實驗結(jié)果
圖注:泳道3:marker
泳道2:0號對照
泳道1:含8M尿素上清
泳道0:bingding buffer 上清
泳道 0 1 2 3
本蛋白形成包涵體,在使用此研磨機后進行上樣電泳。圖譜清晰,說明本研磨機適用于含包涵體菌體的破碎。
Previous:高通量組織研磨儀針對中藥藥材DNA提取的研磨方法
Next:高通量組織研磨儀研磨腫瘤組織提取RNA
Related Products
- Home
- About us
-
Product
-
Grinder for scientific research
Grinder for scientific research
- Enhanced multi-sample tissue grinder
- Enhanced automatic sample rapid grinder
- Enhanced cryomill
- Multi-sample tissue grinding machine
- Automatic sample rapid grinder
- Cryogenic tissue grinder
- Three-dimensional motion grinder
- Single cell suspension preparation instrument
- Freezer tissue grinder
- Liquid nitrogen Cryogenic Grinder
- High Throughput Tissue Grinder
-
Grinder for analysis?and?detection
Grinder for analysis?and?detection
- Hair drug detector
- Handheld Grinder
- QuEChERS pre-processing all-in-one machine
- Soil/High Energy Planetary Grinder
- Hammer cyclone mill
- Knife grinder
- Multifunctional/Automatic Homogenizer
- Three dimensional vibrating ball mill
- Chlorophyll/soil A grinder
- Mortar grinder
- High-efficiency vibrating disc grinder
- Jaw grinder
- Flap homogenizer
- Bead-milling tissue grinder
- Special grinder for African swine fever
- Three-dimensional mixing instrument
- Miniature bead mill
- Forensic bone and tooth ball mill
- Percussion grinder
- Rotary splitter
-
General?Instruments
General?Instruments
- Ultrasonic cell disruptor
- Ultrasonic equipment
- In situ hybridization instrument/n instrument/crosslinking
- instrument Dry type thermostat/constant temperature mixer
- Vortex mixer/nitrogen purging instrument
- PCR instrument/membrane sealing instrument Snow ice
- Microplate constant temperature shaking incubator
- Orbital shaker/micro centrifuge
- Inoculation loop sterilization/indicator incubator
- Solid phase extraction device/low temperature thermostat
- Snow ice maker/viscometer/lyophilizer
- Flip oscillator
- Soil drying box/humidity box
-
Xiaomei Ultrasound Series
Xiaomei Ultrasound Series
-
Vibrating?sieve?shakers
Vibrating?sieve?shakers
-
Foreign distribution products
Foreign distribution products
-
Grinder for scientific research
- Customers
- News
- Videos
- Case
- Cantact