1)時(shí)間:2014/3/10 注:部分請與廠家工程師聯(lián)系
2)樣品:豬肌肉組織
3)儀器:主機(jī),Tissuelyser動(dòng)物組織研磨器(上海凈信實(shí)業(yè)有限公司生產(chǎn))
4)配件:離心管和凈信研磨珠
4)步驟:
a.取肌肉組織樣品,均勻地剪切成少量樣品塊,分別放入若干研磨離心管中。
b.在每個(gè)試管中放入幾顆凈信研磨珠和一些PBS裂解液,分別蓋緊管蓋后,再置入研磨儀的適配器中,將適配器固定在研磨儀中。
c.將Tissuelyser動(dòng)物組織研磨器的時(shí)間和頻率參數(shù)設(shè)置好后,就可以啟動(dòng)研磨儀了。
d.研磨完全停止后,拿出研磨離心管,旋開管蓋,取出研磨好的肌肉組織樣品。
5)結(jié)果:
研磨后的豬肌肉組織成很均細(xì)的泥漿狀,說明用Tissuelyser動(dòng)物組織研磨器研磨豬肌肉組織效果良好。
上海凈信的全自動(dòng)樣品快速研磨儀處理與手工液氮研磨處理后提取 FT 的 DNA、RNA 在濃度、純度上均無顯著性差異 。但手工研磨有較大的樣本損失率,對于DNA提取濃度較低的樣品來說,自動(dòng)研磨儀可以做到樣品零損失,在DNA提取上有著明顯的優(yōu)勢,且手工處理的過程復(fù)雜、易交叉污染,操作所用時(shí)間等方面均不及上海凈信的全自動(dòng)樣品快速研磨儀,因此自動(dòng)研磨儀對于DNA提取工作相對于手工研磨處理有很大優(yōu)勢。
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